Overview

  • Product nameAnti-Tbx6 antibody
    See all Tbx6 primary antibodies
  • Description
    Mouse monoclonal to Tbx6
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 191-300 of Human Tbx6

Properties

Applications

Our Abpromise guarantee covers the use of ab58255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 35 µg/ml.

Target

  • FunctionProbable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.
  • Tissue specificityExpressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.
  • Sequence similaritiesContains 1 T-box DNA-binding domain.
  • Developmental stageExpressed during gastrulation and during a second phase in some adult tissues.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNB 67 antibody
    • DFNB67 antibody
    • SCDO5 antibody
    • T box 6 antibody
    • T box protein 6 antibody
    • T box transcription factor TBX 6 antibody
    • T box transcription factor TBX6 antibody
    • T-box protein 6 antibody
    • T-box transcription factor TBX6 antibody
    • Tbox 6 antibody
    • TBX 6 antibody
    • tbx6 antibody
    • TBX6_HUMAN antibody
    see all

Anti-Tbx6 antibody images

  • Overlay histogram showing HEK293 cells stained with ab58255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58255, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 47 kDa
    Tbx6 antibody (ab58255) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58255 at 35 ug/ml staining Tbx6 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Tbx6 antibody (ab58255)

ab58255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"