Overview

  • Product name
  • Description
    Rabbit polyclonal to TC10
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide between amino acids 55-84 from the central region of Human TC10 (NP_036381.2).

  • Positive control
    • T47D cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab107573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 23 kDa.

Target

  • Function
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Post-translational
    modifications
    May be post-translationally modified by both palmitoylation and polyisoprenylation.
  • Cellular localization
    Cytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHQ antibody
    • Ras homolog gene family member Q antibody
    • RAS like family 7 member A antibody
    • Ras like protein family member 7A antibody
    • Ras like protein TC10 antibody
    • Ras related GTP binding protein TC10 antibody
    • Ras-like protein family member 7A antibody
    • Ras-like protein TC10 antibody
    • RASL7A antibody
    • Rho related GTP binding protein RhoQ antibody
    • Rho-related GTP-binding protein RhoQ antibody
    • Rhoq antibody
    • RHOQ_HUMAN antibody
    • TC10A antibody
    see all

Images

  • Anti-TC10 antibody (ab107573) at 1/100 dilution + T47D cell line lysates at 35 µg

    Predicted band size : 23 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107573 at 1/50.

References

This product has been referenced in:
  • Mayer S  et al. Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission. Proc Natl Acad Sci U S A 110:20795-800 (2013). WB . Read more (PubMed: 24297911) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Baboon Tissue lysate - whole (Fetal Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (11% gel)
Loading amount
20 µg
Specification
Fetal Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Soon-Ok Kim

Verified customer

Submitted Feb 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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