Anti-TC10 antibody (ab168645)
Key features and details
- Mouse polyclonal to TC10
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TC10 antibody
See all TC10 primary antibodies -
Description
Mouse polyclonal to TC10 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein within Human TC10 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P17081 -
Positive control
- TC10 transfected 293T cell lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modificationsMay be post-translationally modified by both palmitoylation and polyisoprenylation. -
Cellular localization
Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 23433 Human
- Entrez Gene: 104215 Mouse
- Entrez Gene: 85428 Rat
- Omim: 605857 Human
- SwissProt: P17081 Human
- SwissProt: Q8R527 Mouse
- SwissProt: Q9JJL4 Rat
- Unigene: 709193 Human
see all -
Alternative names
- ARHQ antibody
- Ras homolog gene family member Q antibody
- RAS like family 7 member A antibody
see all
Images
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All lanes : Anti-TC10 antibody (ab168645) at 1 µg/ml
Lane 1 : TC10 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa -
Immunofluorescence analysis of HeLa cells, labeling TC10 with ab168645 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab168645 has been referenced in 1 publication.
- Mayer S et al. Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission. Proc Natl Acad Sci U S A 110:20795-800 (2013). PubMed: 24297911