Overview

  • Product nameAnti-TCEA1 antibody
    See all TCEA1 primary antibodies
  • Description
    Rabbit polyclonal to TCEA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog, Human
  • Immunogen

    A region within synthetic peptide: SSSNVSSRKD ETNARDTYVS SFPRAPSTSD SVRLKCREML AAALRTGDDY, corresponding to internal sequence amino acids 109-158 of Human TCEA1

  • Positive control
    • SP2\0 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Involvement in diseaseNote=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similaritiesBelongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Anti-TCEA1 antibody images

  • Anti-TCEA1 antibody (ab49176) at 1.25 µg/ml + SP2\0 cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-TCEA1 antibody (ab49176)

ab49176 has not yet been referenced specifically in any publications.

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