The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged TCEA1 is approximately 1ng/ml as a capture antibody.
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Involvement in diseaseNote=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) . Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Sequence similaritiesBelongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
All lanes : Anti-TCEA1 antibody (ab76637) at 1/500 dilution
Lane 1 : TCEA1 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 34 kDa Observed band size : 34 kDa
ELISA - TCEA1 antibody (ab76637)
The antibody concentration should be 10µg/mL in coating buffer (PBS), the volume should be 100µL/well. Blocking solution was added at 250ul per well (5% Skim Milk in PBS + 0.05% Tween-20). Secondary antibody used was HRP conjugated goat anti-rabbit IgG (H+L).
References for Anti-TCEA1 antibody (ab76637)
has not yet been referenced specifically in any publications.
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