Overview

  • Product nameAnti-TCEAL1 antibody
    See all TCEAL1 primary antibodies
  • Description
    Mouse monoclonal to TCEAL1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: MDKPRKENEE EPQSAPKTDE ERPPVEHSPE KQSPEEQSSE EQSSEEEFFP EELLPELLPE MLLSEERPPQ EGLSRKDLFE GRPPMEQPPC GVGKHKLEEG , corresponding to amino acids 1-100 of Human TCEAL1 with tag (NP_001006640)

  • Positive control
    • TCEAL1 transfected 293T cell lysate; Recombinant fragment of human TCEAL1.

Properties

Applications

Our Abpromise guarantee covers the use of ab77661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay be involved in transcriptional regulation. Modulates various viral and cellular promoters in a promoter context-dependent manner. For example, transcription from the FOS promoter is increased, while Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter activity is repressed. Does not bind DNA directly.
  • Tissue specificityExpressed in all tissues examined. Highly expressed in heart, ovary, prostate and skeletal muscle. Moderately expressed in brain, placenta, testis and small intestine. Weakly expressed in lung, liver and spleen. Expressed in several cancer cell lines.
  • Sequence similaritiesBelongs to the TFS-II family. TFA subfamily.
  • Post-translational
    modifications
    Phosphorylation of Ser-36 and Ser-37 is critical for transcriptional repression.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Highly similar to gene pp21 protein [H.sapiens] antibody
    • Nuclear phosphoprotein p21/SIIR antibody
    • p21 antibody
    • pp21 antibody
    • SIIR antibody
    • TCAL1_HUMAN antibody
    • TCEA like protein 1 antibody
    • TCEA-like protein 1 antibody
    • TCEAL1 antibody
    • Transcription elongation factor A (SII) like 1 antibody
    • Transcription elongation factor A protein like 1 antibody
    • Transcription elongation factor A protein-like 1 antibody
    • Transcription elongation factor S II protein like 1 antibody
    • Transcription elongation factor S-II protein-like 1 antibody
    see all

Anti-TCEAL1 antibody images

  • Anti-TCEAL1 antibody (ab77661) at 5 µg/ml + Recombinant fragment of human TCEAL1 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 18 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TCEAL1 antibody (ab77661) at 5 µg/ml

    Lane 1 : TCEAL1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 18 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-TCEAL1 antibody (ab77661)

ab77661 has not yet been referenced specifically in any publications.

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