Recombinant
RabMAb

Anti-TCEB2 antibody [EPR10441(B)] (ab154854)

Overview

  • Product name
    Anti-TCEB2 antibody [EPR10441(B)]
    See all TCEB2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10441(B)] to TCEB2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human TCEB2 (UniProt: Q15370).

  • Positive control
    • Jurkat, A431, K562 cell lysates; Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
      The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 ubiquitin-like domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EloB antibody
      • ELOB_HUMAN antibody
      • Elongin 18 kDa subunit antibody
      • Elongin B antibody
      • Elongin-B antibody
      • RNA polymerase II transcription factor SIII p18 subunit antibody
      • RNA polymerase II transcription factor SIII subunit B antibody
      • SIII antibody
      • SIII p18 antibody
      • TCEB 2 antibody
      • Tceb2 antibody
      • Transcription elongation factor B (SIII) polypeptide 2 antibody
      • Transcription elongation factor B polypeptide 2 antibody
      see all

    Images

    • All lanes : Anti-TCEB2 antibody [EPR10441(B)] (ab154854) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 13 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling TCEB2 with ab154854 at 1/10 dilution (red). Rabbit IgG negative control (green).

    References

    ab154854 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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