The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
TCEB3B is the transcriptionally active subunit of the SIII (or elongin) transcription elongation factor complex, which comprehends also two regulatory subunits (elongins B and C). It increases the RNA polymerase II transcription elongation past template-encoded arresting sites the encoded This protein is specifically expressed in the testis, suggesting a possible role in spermatogenesis.