Overview

  • Product nameAnti-TCF12 antibody
    See all TCF12 primary antibodies
  • Description
    Rabbit polyclonal to TCF12
  • Tested applicationsSuitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human TCF12 (NP_996919.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionBinds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems.
  • Tissue specificityExpressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb20 antibody
    • Class B basic helix-loop-helix protein 20 antibody
    • DNA binding protein HTF4 antibody
    • DNA-binding protein HTF4 antibody
    • E box binding protein antibody
    • E-box-binding protein antibody
    • HEB antibody
    • Helix loop helix transcription factor 4 antibody
    • HsT17266 antibody
    • HTF4 antibody
    • HTF4_HUMAN antibody
    • TCF-12 antibody
    • Tcf12 antibody
    • Transcription factor 12 antibody
    • Transcription factor HTF-4 antibody
    • Transcription factor HTF4 antibody
    see all

Anti-TCF12 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TCF12 with ab70746 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TCF12 antibody (ab70746) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 73 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,120 kDa,190 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • 1mg whole cell lysate from HeLa cells was immunprecipitated with ab70746 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab707345 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds

References for Anti-TCF12 antibody (ab70746)

ab70746 has not yet been referenced specifically in any publications.

Product Wall

The E2A has about a 60% chance of reactivity with this antibody and the E2-2 has about a 30% of reactivity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"