Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5% Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    Peptide ELISA: antibody detection limit dilution 1/32000
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 41 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potential trans-activating factor that could play an important role in the transcription of genes required for the later stages of cell cycle progression.
    • Sequence similarities
      Contains 1 FHA domain.
      Contains 1 PHD-type zinc finger.
    • Developmental stage
      Growth regulated.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • SC 1 antibody
      • SC1 antibody
      • TCF 19 antibody
      • TCF-19 antibody
      • Tcf19 antibody
      • TCF19_HUMAN antibody
      • Transcription factor 19 antibody
      • Transcription factor SC1 antibody
      see all

    Images



    • Predicted band size : 41 kDa


      ab5344 staining (1.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References

    This product has been referenced in:
    • Matsumoto T  et al. Small Heat Shock Protein Beta-1 (HSPB1) Is Upregulated and Regulates Autophagy and Apoptosis of Renal Tubular Cells in Acute Kidney Injury. PLoS One 10:e0126229 (2015). Read more (PubMed: 25962073) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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