Overview

  • Product nameAnti-Tcf20 antibody
  • Description
    Rabbit polyclonal to Tcf20
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1909-1958 (HYPCAIDADC LLHEENFSVR CPKHKPPLPC PLPPLQNKTA KGSLSTEQSE) of Human Tcf20 (NP_005641).

  • Positive control
    • OVCAR3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab112089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 212 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceTcf20 is thought to be a transcriptional coactivator, enhancing the activity of transcription factors such as JUN and SP1. Alternative splicing results in two transcript variants encoding different isoforms.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AR1 antibody
    • KIAA0292 antibody
    • Nuclear factor SPBP antibody
    • Protein AR1 antibody
    • SPBP antibody
    • Stromelysin 1 PDGF-responsive element-binding protein antibody
    • TCF-20 antibody
    • Tcf20 antibody
    • Transcription factor 20 antibody
    see all

Anti-Tcf20 antibody images

  • Anti-Tcf20 antibody (ab112089) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Predicted band size : 212 kDa

References for Anti-Tcf20 antibody (ab112089)

ab112089 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Lung endothelial cells)
Specification Lung endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% triton x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (immortalized endothelial cells)
Loading amount 5 µg
Specification immortalized endothelial cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"