Overview

  • Product nameAnti-TCF23 antibody
  • Description
    Rabbit polyclonal to TCF23
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    A region within synthetic peptide: RLYAGGLGCS DLDSTTAITT GQRCKDAELG SQDSVAAESL LTSPAFGNK, corresponding to C terminal amino acids 161-209 of Human TCF23

  • Positive control
    • SP2\0 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:62500.

Target

  • FunctionInhibits E-box-mediated binding and transactivation of bHLH factors. Inhibitory effect is similar to that of ID proteins. Inhibits the formation of TCF3 and MYOD1 homo- and heterodimers. Lacks DNA binding activity. Seems to play a role in the inhibition of myogenesis.
  • Tissue specificityExpressed in liver, kidney and spleen.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • DomainBoth the bHLH region and the C-terminal portion are essential for inhibitory function.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHa24 antibody
    • Class A basic helix-loop-helix protein 24 antibody
    • Class II basic helix loop helix protein antibody
    • OUT antibody
    • TCF-23 antibody
    • TCF23 antibody
    • TCF23_HUMAN antibody
    • Transcription factor 23 antibody
    see all

Anti-TCF23 antibody images

  • Anti-TCF23 antibody (ab49179) at 2.5 µg/ml + SP2\0 cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 23 kDa
    Observed band size : 23 kDa

References for Anti-TCF23 antibody (ab49179)

ab49179 has not yet been referenced specifically in any publications.

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