• Product nameAnti-TCF3 / E2A antibody
    See all TCF3 / E2A primary antibodies
  • Description
    Goat polyclonal to TCF3 / E2A
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from an internal region:


    by a Cysteine residue linker, corresponding to amino acids 522-535 of Human TCF3/ E2A

  • Positive control
    • Daudi cell lysate



Our Abpromise guarantee covers the use of ab59117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/128000.


  • FunctionHeterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
  • Involvement in diseaseNote=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    Phosphorylated following NGF stimulation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGM8 antibody
    • bHLHb21 antibody
    • Class B basic helix-loop-helix protein 21 antibody
    • E12 antibody
    • E2A antibody
    • E2A immunoglobulin enhancer binding factors E12/E47 antibody
    • E47 antibody
    • Helix loop helix protein HE47 antibody
    • Immunoglobulin enhancer-binding factor E12/E47 antibody
    • Immunoglobulin transcription factor 1 antibody
    • ITF1 antibody
    • Kappa-E2-binding factor antibody
    • MGC129647 antibody
    • MGC129648 antibody
    • Negative vitamin D response element binding protein antibody
    • NOL1-TCF3 fusion antibody
    • TCF-3 antibody
    • Tcf3 antibody
    • TFE2_HUMAN antibody
    • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
    • Transcription factor 3 antibody
    • transcription factor 3 variant 3 antibody
    • Transcription factor E2-alpha antibody
    • Transcription factor ITF-1 antibody
    • VDIR antibody
    • VDR interacting repressor antibody
    • vitamin D receptor-interacting repressor antibody
    see all

Anti-TCF3 / E2A antibody images

  • Anti-TCF3 / E2A antibody (ab59117) at 0.01 µg/ml + Daudi cell lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab59117 (3.8 µg/ml) staining TCF3/E2A in paraffin-embedded human kidney tissue following steamed antigen retrival with citrate buffer (pH 6).

References for Anti-TCF3 / E2A antibody (ab59117)

ab59117 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem Cells)
Permeabilization Yes - 0.2% TritonX
Specification Embryonic Stem Cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde

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Submitted Aug 10 2016