The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
1/50 - 1/100.
FunctionHeterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Involvement in diseaseNote=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
References for Anti-TCF3 / E2A antibody (ab69999)
This product has been referenced in:
Li C et al. Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS One9:e112005 (2014).
Read more (PubMed: 25375219) »
Lee K et al. Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. EMBO J30:2662-74 (2011).
Read more (PubMed: 21610693) »