Anti-TCF3 / E2A antibody (ab69999)
- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-TCF3 / E2A antibody
See all TCF3 / E2A primary antibodies - DescriptionRabbit polyclonal to TCF3 / E2A
- SpecificityDetects endogenous levels of total TCF3/E2A protein.
- Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Immunogen
Synthesized peptide derived from the C terminus of human TCF3/E2A
- Positive control
- WB: Extracts from 293 cells IHC-P: Human brain tissue
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAffinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab69999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| ELISA | 1/20000. | |
| IHC-P | 1/50 - 1/100. |
Target
- FunctionHeterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
- Involvement in diseaseNote=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
- Post-translational
modificationsPhosphorylated following NGF stimulation. - Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 6929 Human
- Entrez Gene: 21423 Mouse
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P15806 Mouse
- SwissProt: P21677 Rat
- Unigene: 371282 Human
see all -
Alternative names
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
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Anti-TCF3 / E2A antibody images
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Western blot - TCF3 / E2A antibody (ab69999)All lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution
Lane 1 : Extracts from 293 cells, minus immunising peptide
Lane 2 : Extracts from 293 cells, plus immunising peptide 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 68 kDa
Observed band size : 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF3 / E2A antibody (ab69999)ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
References for Anti-TCF3 / E2A antibody (ab69999)
This product has been referenced in:
- Li C et al. Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS One 9:e112005 (2014). IHC ; Human . Read more (PubMed: 25375219) »
- Lee K et al. Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. EMBO J 30:2662-74 (2011). WB, IF ; Mouse . Read more (PubMed: 21610693) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"