Overview

  • Product name
    Anti-TCF3 / E2A antibody
    See all TCF3 / E2A primary antibodies
  • Description
    Rabbit polyclonal to TCF3 / E2A
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of total TCF3/E2A protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized peptide derived from the C terminus of human TCF3/E2A

  • Positive control
    • WB: Extracts from 293 cells IHC-P: Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab69999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
  • Involvement in disease
    Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Phosphorylated following NGF stimulation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AGM8 antibody
    • bHLHb21 antibody
    • Class B basic helix-loop-helix protein 21 antibody
    • E12 antibody
    • E2A antibody
    • E2A immunoglobulin enhancer binding factors E12/E47 antibody
    • E47 antibody
    • Helix loop helix protein HE47 antibody
    • Immunoglobulin enhancer-binding factor E12/E47 antibody
    • Immunoglobulin transcription factor 1 antibody
    • ITF1 antibody
    • Kappa-E2-binding factor antibody
    • MGC129647 antibody
    • MGC129648 antibody
    • Negative vitamin D response element binding protein antibody
    • NOL1-TCF3 fusion antibody
    • TCF-3 antibody
    • Tcf3 antibody
    • TFE2_HUMAN antibody
    • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
    • Transcription factor 3 antibody
    • transcription factor 3 variant 3 antibody
    • Transcription factor E2-alpha antibody
    • Transcription factor ITF-1 antibody
    • VDIR antibody
    • VDR interacting repressor antibody
    • vitamin D receptor-interacting repressor antibody
    see all

Images

  • Western blot - TCF3 / E2A antibody (ab69999)
    Western blot - TCF3 / E2A antibody (ab69999)
    All lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, minus immunising peptide
    Lane 2 : Extracts from 293 cells, plus immunising peptide 5µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF3 / E2A antibody (ab69999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF3 / E2A antibody (ab69999)
    ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).

References

This product has been referenced in:
  • Loriot C  et al. Deciphering the molecular basis of invasiveness in Sdhb-deficient cells. Oncotarget 6:32955-65 (2015). WB . Read more (PubMed: 26460615) »
  • Li C  et al. Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS One 9:e112005 (2014). IHC ; Human . Read more (PubMed: 25375219) »

See all 3 Publications for this product

Publishing research using ab69999? Please let us know so that we can cite the reference in this datasheet.

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