Overview

  • Product nameAnti-TCF3 / E2A antibody
    See all TCF3 / E2A primary antibodies
  • Description
    Rabbit polyclonal to TCF3 / E2A
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH.

  • Positive control
    • Primary Human keratinocyte cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/8000 is determined for one of the three peptides.
    WB: 1/200. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHeterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
    • Involvement in diseaseNote=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • Post-translational
      modifications
      Phosphorylated following NGF stimulation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGM8 antibody
      • bHLHb21 antibody
      • Class B basic helix-loop-helix protein 21 antibody
      • E12 antibody
      • E2A antibody
      • E2A immunoglobulin enhancer binding factors E12/E47 antibody
      • E47 antibody
      • Helix loop helix protein HE47 antibody
      • Immunoglobulin enhancer-binding factor E12/E47 antibody
      • Immunoglobulin transcription factor 1 antibody
      • ITF1 antibody
      • Kappa-E2-binding factor antibody
      • MGC129647 antibody
      • MGC129648 antibody
      • Negative vitamin D response element binding protein antibody
      • NOL1-TCF3 fusion antibody
      • TCF-3 antibody
      • Tcf3 antibody
      • TFE2_HUMAN antibody
      • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) antibody
      • Transcription factor 3 antibody
      • transcription factor 3 variant 3 antibody
      • Transcription factor E2-alpha antibody
      • Transcription factor ITF-1 antibody
      • VDIR antibody
      • VDR interacting repressor antibody
      • vitamin D receptor-interacting repressor antibody
      see all

    Anti-TCF3 / E2A antibody images

    • Anti-TCF3 / E2A antibody (ab82847) at 1/200 dilution + Primary Human keratinocyte cell lysate at 30 µg

      Secondary
      anti-Rabbit IgG HRP at 1000

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    References for Anti-TCF3 / E2A antibody (ab82847)

    ab82847 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
    Loading amount 50 µg
    Specification Mouse ES Cells
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Utkarsh Kapoor

    Verified customer

    Submitted Dec 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"