Anti-TCF3 / E2A (phospho T355) antibody (ab85772)
Key features and details
- Rabbit polyclonal to TCF3 / E2A (phospho T355)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCF3 / E2A (phospho T355) antibody
See all TCF3 / E2A primary antibodies -
Description
Rabbit polyclonal to TCF3 / E2A (phospho T355) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Dog -
Immunogen
Synthetic peptide corresponding to Human TCF3/ E2A aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103576) -
Positive control
- This antibody gave a positive signal in both HeLa and HepG2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. -
Involvement in disease
Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsPhosphorylated following NGF stimulation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6929 Human
- Entrez Gene: 21423 Mouse
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P15806 Mouse
- SwissProt: P21677 Rat
- Unigene: 371282 Human
see all -
Alternative names
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
see all
Images
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All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85772 has not yet been referenced specifically in any publications.