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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).
Function Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
Involvement in disease Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.
Sequence similarities Contains 1 basic helix-loop-helix (bHLH) domain.
Domain the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Post-translational modifications Phosphorylated following NGF stimulation.
Cellular localization Nucleus.
Information by UniProt
Class B basic helix-loop-helix protein 21 antibody
Anti-TCF3 / E2A (phospho T355) antibody images
Western blot - TCF3 / E2A (phospho T355) antibody (ab85772)
All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide ( ab118180) at 1 µg/ml Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide ( ab118180) at 1 µg/ml Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide ( ab103576) at 1 µg/ml Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide ( ab103576) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 68 kDa Observed band size : 80 kDa ( why is the actual band size different from the predicted?) Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
References for Anti-TCF3 / E2A (phospho T355) antibody (ab85772)
has not yet been referenced specifically in any publications.
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