Overview

  • Product nameAnti-TCF4 antibody
    See all TCF4 primary antibodies
  • Description
    Rabbit polyclonal to TCF4
  • SpecificityDetects endogenous levels of total TCF4 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human TCF4 aa 581-630 (internal sequence).
    Database link: P15884
    (Peptide available as ab101669)

  • Positive control
    • HUVEC extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ELISA 1/20000.

Target

  • FunctionTranscription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
  • Tissue specificityExpressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
  • Involvement in diseaseDefects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb19 antibody
    • Class B basic helix-loop-helix protein 19 antibody
    • E2 2 antibody
    • Immunoglobulin transcription factor 2 antibody
    • ITF 2 antibody
    • ITF-2 antibody
    • ITF2 antibody
    • ITF2_HUMAN antibody
    • MGC149723 antibody
    • MGC149724 antibody
    • PTHS antibody
    • SEF 2 antibody
    • SEF-2 antibody
    • SEF2 1 antibody
    • SEF2 1A antibody
    • SEF2 1B antibody
    • SEF2 antibody
    • SL3 3 enhancer factor 2 antibody
    • SL3-3 enhancer factor 2 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • Transcription factor 4 antibody
    see all

Anti-TCF4 antibody images

  • All lanes : Anti-TCF4 antibody (ab72586) at 1/500 dilution

    Lane 1 : HUVEC extract
    Lane 2 : HUVEC extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 71 kDa
    Observed band size : 71 kDa

References for Anti-TCF4 antibody (ab72586)

This product has been referenced in:
  • Lemieux ME  et al. Inactivation of a Single Copy of Crebbp Selectively Alters Pre-mRNA Processing in Mouse Hematopoietic Stem Cells. PLoS One 6:e24153 (2011). WB ; Mouse . Read more (PubMed: 21901164) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"