• Product name
  • Description
    Rabbit polyclonal to TCF7
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from residues 228 - 277 of human Transcription factor 7.


  • Positive control
    • WB: fetal thymus lysate. IHC-P: human kidney tissue.



Our Abpromise guarantee covers the use of ab30961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • Function
    Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
  • Tissue specificity
    Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
  • Sequence similarities
    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36364 antibody
    • MGC47735 antibody
    • OTTHUMP00000159391 antibody
    • T cell factor 1 antibody
    • T cell specific transcription factor 1 antibody
    • T-cell factor 1 antibody
    • T-cell-specific transcription factor 1 antibody
    • TCF-1 antibody
    • TCF-7 antibody
    • TCF1 antibody
    • Tcf7 antibody
    • TCF7 transcription factor 7 antibody
    • TCF7_HUMAN antibody
    • Transcription factor 7 (T cell specific HMG box) antibody
    • Transcription factor 7 antibody
    • Transcription factor-7, T-cell specific antibody
    • Transcription factor-7, T-cell specific, 1 antibody
    see all

Anti-TCF7 antibody images

  • Anti-TCF7 antibody (ab30961) at 0.125 µg/ml + fetal thymus lysate at 10 µg/ml

    HRP conjugated anti rabbit IgG at a dilution of 1/ 50,000 - 1/100,000
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed parraffin-embedded sections) analysis of human kidney tissue labelling Transcription factor 7 with ab30961 at 4-8µg/ml. Positively labelled cells (epithelial cells of renal tubule) are indicated by arrows. Magnification: 400X.

References for Anti-TCF7 antibody (ab30961)

This product has been referenced in:
  • Li W  et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). Read more (PubMed: 25644173) »

See 1 Publication for this product

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