Anti-TCF7 antibody (ab58271)

Overview

  • Product nameAnti-TCF7 antibody
    See all TCF7 primary antibodies
  • Description
    Mouse monoclonal to TCF7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: VIAECTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK RRSREKHQES TTGGKRNAFG TYPEKAAAPA PFLPMTVL, corresponding to amino acids 287-385 of Human TCF7

Properties

Applications

Our Abpromise guarantee covers the use of ab58271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
    • Tissue specificityPredominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
    • Sequence similaritiesBelongs to the TCF/LEF family.
      Contains 1 HMG box DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ36364 antibody
      • MGC47735 antibody
      • OTTHUMP00000159391 antibody
      • T cell factor 1 antibody
      • T cell specific transcription factor 1 antibody
      • T-cell factor 1 antibody
      • T-cell-specific transcription factor 1 antibody
      • TCF-1 antibody
      • TCF-7 antibody
      • TCF1 antibody
      • Tcf7 antibody
      • TCF7 transcription factor 7 antibody
      • TCF7_HUMAN antibody
      • Transcription factor 7 (T cell specific HMG box) antibody
      • Transcription factor 7 antibody
      • Transcription factor-7, T-cell specific antibody
      • Transcription factor-7, T-cell specific, 1 antibody
      see all

    Anti-TCF7 antibody images

    • TCF7 antibody (ab58271) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
    • Western blot against tagged recombinant protein immunogen using ab58271 TCF7 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-TCF7 antibody (ab58271)

    ab58271 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (Skeleton)
    Specification Skeleton
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 12 2014

    Thank you for contacting Abcam.


    I have looked at both ab58271 and ab90136 and although they have not been tested on mouse tissue, based on sequence homology there is a high chance that both of these antibodies will react to mouse tissue...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"