Overview

  • Product nameAnti-TCF7 antibody
    See all TCF7 primary antibodies
  • Description
    Rabbit polyclonal to TCF7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 216 - 265 (QLYPGWSARD NYGKKKRRSR EKHQESTTET NWPRELKDGN GQESLSMSSS) of Human TCF7 isoform 3 (NP_963964).

  • Positive control
    • Fetal small intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab90136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
  • Tissue specificityPredominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
  • Sequence similaritiesBelongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36364 antibody
    • MGC47735 antibody
    • OTTHUMP00000159391 antibody
    • T cell factor 1 antibody
    • T cell specific transcription factor 1 antibody
    • T-cell factor 1 antibody
    • T-cell-specific transcription factor 1 antibody
    • TCF-1 antibody
    • TCF-7 antibody
    • TCF1 antibody
    • Tcf7 antibody
    • TCF7 transcription factor 7 antibody
    • TCF7_HUMAN antibody
    • Transcription factor 7 (T cell specific HMG box) antibody
    • Transcription factor 7 antibody
    • Transcription factor-7, T-cell specific antibody
    • Transcription factor-7, T-cell specific, 1 antibody
    see all

Anti-TCF7 antibody images

  • Anti-TCF7 antibody (ab90136) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa

References for Anti-TCF7 antibody (ab90136)

ab90136 has not yet been referenced specifically in any publications.

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I have looked at both ab58271 and ab90136 and although they have not been tested on mouse tissue, based on sequence homology there is a high chance that both of these antibodies will react to mouse tissue...

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