Anti-TCF7L1 antibody (ab86175)

Overview

  • Product nameAnti-TCF7L1 antibody
    See all TCF7L1 primary antibodies
  • Description
    Rabbit polyclonal to TCF7L1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 36-85 (DELIPFQDEG GEEQEPSSDS ASAQRDLDEV KSSLVNESEN QSSSSDSEAE) of Human TCF7L1, NP_112573

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionParticipates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
  • Tissue specificityDetected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
  • Sequence similaritiesBelongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • DomainThe putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb21 antibody
    • HMG box transcription factor 3 antibody
    • HMG box transcription factor antibody
    • OTTMUSP00000023419 antibody
    • T cell factor 3 antibody
    • TCF 3 antibody
    • TCF-3 antibody
    • TCF3 antibody
    • Tcf7l1 antibody
    • TF7L1_HUMAN antibody
    • Transcription factor 7 like 1 (T-cell specific, HMG-box) antibody
    • Transcription factor 7 like 1 antibody
    • Transcription factor 7-like 1 antibody
    see all

Anti-TCF7L1 antibody images

  • Anti-TCF7L1 antibody (ab86175) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,46 kDa,>90 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling TCF7L1 with ab86175 at 5.0µg/ml.

References for Anti-TCF7L1 antibody (ab86175)

ab86175 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Loading amount 50000 cells
Specification mouse embryonic stem cells
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Mar 02 2012

Thank you very much for your interest. To our knowledge, these two antibodies have not been tested in Mouse and  IP. We only have data in WB  on human samples. Therefore, I can offer a discount off a future purchase if you buy either of them now, t...

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For ab82960: DISCOUNT CODE: *** For ab86175: DISCOUNT CODE: *** Expiration date: March 1st, 2012 I am very pleased to hear you would like to accept our offer and test these antibodies in mouse. Each code will give you 1 free primary antibody before the...

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Thank you very much for your interest in ab82690 and ab86175. To our knowledge, neither of these antibodies have been tested on mouse samples.  Unfortunately we do not offer free or trial sized samples, however, I can offer a discount of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"