Overview

  • Product nameAnti-TCF7L2 antibody
    See all TCF7L2 primary antibodies
  • Description
    Rabbit polyclonal to TCF7L2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids 252-301 (GFRHPYPTAL TVNASVSRFP PHMVPPHHTL HTTGIPHPAI VTPTVKQESS) of Human T cell Transcription Factor 4 (NP_110383)

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1.777777778

Target

  • FunctionParticipates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
  • Tissue specificityDetected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.
  • Involvement in diseaseNote=Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
    Genetic variations in TCF7L2 are associated with susceptibility to non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similaritiesBelongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Developmental stageHighly expressed in crypt regions and barely detectable in villi in epithelium from fetal small intestine at week 16. At week 22 expression in villi had increased strongly.
  • DomainThe promoter-specific activation domain interacts with the transcriptional coactivator EP300.
  • Post-translational
    modifications
    In vitro, phosphorylated by TNIK.
    Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylated by AXAM/SENP2. Sumoylation/desumoylation regulates TCF4 transcription activity in the Wnt signaling pathway without altering interaction with CTNNB1 nor binding DNA.
  • Cellular localizationNucleus > PML body. Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • HMG box transcription factor 4 antibody
    • hTCF 4 antibody
    • hTCF-4 antibody
    • T cell factor 4 antibody
    • T cell specific HMG box antibody
    • T cell specific transcription factor 4 antibody
    • T-cell factor 4 antibody
    • T-cell-specific transcription factor 4 antibody
    • TCF 4 antibody
    • TCF-4 antibody
    • TCF4 antibody
    • TCF7L2 antibody
    • TCF7L2 protein antibody
    • TF7L2_HUMAN antibody
    • Transcription factor 7 like 2 antibody
    • Transcription factor 7 like 2 T cell specific HMG box antibody
    • Transcription factor 7-like 2 antibody
    see all

Anti-TCF7L2 antibody images



  • Predicted band size : 68 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling TCF7L2 with ab82768 at 1.0µg/ml.


  • Predicted band size : 68 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling TCF7L2 with ab82768 at 1.0µg/ml.


  • Predicted band size : 68 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling TCF7L2 with ab82768 at 1.0µg/ml.
  • Anti-TCF7L2 antibody (ab82768) at 1 µg/ml + Human fetal muscle lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

References for Anti-TCF7L2 antibody (ab82768)

ab82768 has not yet been referenced specifically in any publications.

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