Synthetic peptide. within Human TCF7L2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9NQB0
Alternative versions available:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab76151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25000 - 1/100000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/100 - 1/250.|
Immunofluorescence staining of HT-29 cells with purified ab76151 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.