Overview

  • Product nameAnti-TCFL5 antibody
    See all TCFL5 primary antibodies
  • Description
    Goat polyclonal to TCFL5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-KYIQERHGDSLKKE

    , corresponding to internal sequence amino acids 400-413 of Human TCFL5

  • Positive control
    • Human Testis lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab53546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPutative transcription factor. Isoform 3 may play a role in early spermatogenesis.
    • Tissue specificityIsoform 3 is testis specific. Isoform 2 is pancreas specific.
    • Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
    • Developmental stageIsoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHe82 antibody
      • CHA antibody
      • Cha transcription factor antibody
      • E2BP-1 antibody
      • E2BP1 antibody
      • Figlb antibody
      • HPV-16 E2-binding protein 1 antibody
      • MGC46135 antibody
      • TCFL 5 antibody
      • TCFL5 antibody
      • TCFL5_HUMAN antibody
      • transcription factor-like 5 (basic helix-loop-helix) antibody
      • Transcription factor-like 5 protein antibody
      see all

    Anti-TCFL5 antibody images

    • All lanes : ab53546 antibody (0.3µg/ml) staining of TCFL5 in Human Testis lysate (35µg protein in RIPA buffer).

      Lane 1 : Without the immunising peptide
      Lane 2 : With the immunising peptide


      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-TCFL5 antibody (ab53546)

    ab53546 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"