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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Affinity-purified from rabbit antiserum by affinity-chromatography using
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
Function Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.
Tissue specificity Isoform 3 is testis specific. Isoform 2 is pancreas specific.
Sequence similarities Contains 1 bHLH (basic helix-loop-helix) domain.
Developmental stage Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids.
Cellular localization Nucleus.
Information by UniProt
Cha transcription factor antibody
Anti-TCFL5 antibody images
Western blot - TCFL5 antibody (ab69931)
All lanes : Anti-TCFL5 antibody (ab69931) at 1/500 dilution Lane 1 : RAW264.7 cell extract Lane 2 : RAW264.7 cell extract with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 53 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?) Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TCFL5 antibody (ab69931)
has not yet been referenced specifically in any publications.
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