Overview

  • Product nameAnti-TCHP antibody
    See all TCHP primary antibodies
  • Description
    Rabbit polyclonal to TCHP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human TCHP.

  • Positive control
    • This antibody gave a positive signal in human tonsil tissue lysate and in the whole cell lysate: U87MG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).

Target

  • RelevanceA tumour suppressor protein which may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery.
  • Cellular localizationCytoplasmic, cytoskeleton
  • Database links
  • Alternative names
    • TCHP antibody
    • Trichoplein keratin filament-binding protein antibody
    • Tumor suppressor protein antibody

Anti-TCHP antibody images

  • All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml

    Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-TCHP antibody (ab77622) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human skin tissue lysate - total protein (ab30166)
    Lane 4 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 5 : T47D whole cell lysate (ab14899)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab77622 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of TCHP staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 77622, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-TCHP antibody (ab77622)

ab77622 has not yet been referenced specifically in any publications.

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The anti-TCHP antibody (ab102638) has currently not been tested for cross reactivity with any other species other than human. The immunogen used to raise the antibody is taken fro...

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