Recombinant Anti-Tcl1 antibody [EPR3949] (ab108978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3949] to Tcl1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Tcl1 antibody [EPR3949]
See all Tcl1 primary antibodies -
Description
Rabbit monoclonal [EPR3949] to Tcl1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Daudi and ramos cell lysates Human tonsil tissue Ramos whole cell lysates Jurkat (human acute T cell leukemia)
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3949 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/400.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified format: 1/100 - 1/1000. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
|
|
IHC-P | (2) |
1/100 - 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform antigen retrieval |
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
Flow Cyt (Intra)
1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified format: 1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
IHC-P
1/100 - 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Perform antigen retrieval |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. -
Tissue specificity
Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. -
Involvement in disease
Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci. -
Sequence similarities
Belongs to the TCL1 family. -
Cellular localization
Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction. - Information by UniProt
-
Database links
- Entrez Gene: 8115 Human
- Omim: 186960 Human
- SwissProt: P56279 Human
- Unigene: 2484 Human
-
Alternative names
- Anti TCL1A antibody
- Lymphoma/leukemia, T-cell antibody
- Oncogene TCL-1 antibody
see all
Images
-
All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 13 kDa
Additional bands at: 13 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
-
ab108978 staining Tcl1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
-
ab108978 staining Tcl1 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab108978 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-goat. at 1/2000 dilution
Predicted band size: 13 kDa -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 showing negative staining in Normal stomach tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab108978 staining Tcl1 in the human cell line Ramos (human Burkitt's lymphoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
-
Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab108978 has been referenced in 1 publication.
- Punt S et al. Whole-transcriptome analysis of flow-sorted cervical cancer samples reveals that B cell expressed TCL1A is correlated with improved survival. Oncotarget 6:38681-94 (2015). PubMed: 26299617