Anti-Tcl1 antibody [EPR3949] (ab108978)

Overview

  • Product nameAnti-Tcl1 antibody [EPR3949]
    See all Tcl1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3949] to Tcl1
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Tcl1 aa 1-100.

  • Positive control
    • Daudi and ramos cell lysates Human tonsil tissue Ramos whole cell lysates Jurkat (human acute T cell leukemia)
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/100 - 1/700. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform antigen retrieval

ICC 1/100 - 1/250.
Flow Cyt 1/400.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Unpurified format: 1/100 - 1/1000.

Target

  • FunctionEnhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
  • Tissue specificityRestricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation.
  • Involvement in diseaseNote=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci.
  • Sequence similaritiesBelongs to the TCL1 family.
  • Cellular localizationCytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anti TCL1A antibody
    • Lymphoma/leukemia, T-cell antibody
    • Oncogene TCL-1 antibody
    • Oncogene TCL1 antibody
    • P14 TCL1 antibody
    • P14 TCL1 protein antibody
    • Protein p14 TCL1 antibody
    • T cell leukemia 1 antibody
    • T cell lymphoma 1 antibody
    • T cell lymphoma 1A antibody
    • T-cell leukemia/lymphoma protein 1A antibody
    • TCL 1 protein antibody
    • TCL1 antibody
    • TCL1 oncogene antibody
    • TCL1 PEN antibody
    • Tcl1a antibody
    • TCL1A antibody antibody
    • TCL1A_HUMAN antibody
    see all

Anti-Tcl1 antibody [EPR3949] images

  • All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma) whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 13 kDa
    Additional bands at : 13 kDa. We are unsure as to the identity of these extra bands.

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

  • ab108978 staining Tcl1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • ab108978 staining Tcl1 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab108978 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • All lanes : Anti-Tcl1 antibody [EPR3949] (ab108978) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : Ramos cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Standard HRP labelled goat anti-goat. at 1/2000 dilution

    Predicted band size : 13 kDa
  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.
  • ab108978 showing negative staining in Skeletal muscle tissue.

  • ab108978 showing positive staining in Normal spleen tissue.

  • ab108978 showing negative staining in Normal brain tissue.

  • ab108978 showing negative staining in Normal kidney tissue.

  • ab108978 showing negative staining in Normal stomach tissue.

  • ab108978 staining Tcl1 in the human cell line Ramos (human Burkitt's lymphoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Tcl1 antibody [EPR3949] (ab108978)

ab108978 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 24°C
Sample Human Tissue sections (Lymph Node)
Specification Lymph Node
Permeabilization Yes - TritonX
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 26 2013

Thank you for contacting us regarding ab108978 and ab91211. I've attached the recommended Abcam IHC-P protocol and a troubleshooting guide for you. Here is a link to the protocol as well: http://www.abcam.com/index.html?pageconfig=resource&rid=11384 ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Oct 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"