Overview

  • Product nameAnti-TCP1 alpha antibody [23c]
    See all TCP1 alpha primary antibodies
  • Description
    Rat monoclonal [23c] to TCP1 alpha
  • SpecificityThis antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human Hsp60 protein.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Dog
  • Immunogen

    Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli.

  • EpitopeAb13501 recognises the COOH group, addition of an alanine (LDDACOOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
  • Positive control
    • RK13 heat shocked cell lysate.
  • General notesAb13501 binds protein A and is useful for native precipitations of coatomer and CTT.

Properties

Applications

Our Abpromise guarantee covers the use of ab13501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 60 kDa.
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similaritiesBelongs to the TCP-1 chaperonin family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI528772 antibody
    • c-cpn antibody
    • CCT alpha antibody
    • CCT antibody
    • CCT-alpha antibody
    • CCT1 antibody
    • Ccta antibody
    • CCTalpha antibody
    • D6S230E antibody
    • MGC133746 antibody
    • p63 antibody
    • T complex 1 antibody
    • T complex protein 1 alpha subunit antibody
    • T complex protein 1 antibody
    • T-complex homolog TCP1 antibody
    • T-complex protein 1 subunit alpha antibody
    • T-complex protein 1 subunit alpha B antibody
    • Tailless complex polypeptide 1 antibody
    • Tailless complex polypeptide 1A antibody
    • Tailless complex polypeptide 1B antibody
    • TCP 1 alpha antibody
    • Tcp-1 antibody
    • TCP-1-alpha antibody
    • TCP1 antibody
    • TCPA_HUMAN antibody
    • Tp63 antibody
    • TRic antibody
    see all

References for Anti-TCP1 alpha antibody [23c] (ab13501)

This product has been referenced in:
  • Lobanova ES  et al. Proteasome overload is a common stress factor in multiple forms of inherited retinal degeneration. Proc Natl Acad Sci U S A 110:9986-91 (2013). Mouse . Read more (PubMed: 23716657) »

See 1 Publication for this product

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"