SpecificityThis antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92kD on immunoblots.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at a concentration of 5 µg/ml.
IP: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 [ECL] or 2 [Colorimetric] µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 67 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.