Overview

  • Product nameAnti-TCP1 alpha antibody [91a]
    See all TCP1 alpha primary antibodies
  • Description
    Rat monoclonal [91a] to TCP1 alpha
  • SpecificityCross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Monkey
    Predicted to work with: Plants
  • Immunogen

    Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).

  • EpitopeThis affinity purified antibody recognizes an epitope in the C terminus of mouse TCP1 alpha (465-AKLRA).
  • Positive control
    • HeLa, PC12 and 3T3 and RK-13 cell lysates (all heat shocked). This antibody gave a positive result in IHC in the following FFPE tissue: Human testis seminoma.
  • General notes


    ab90357 reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, ab90357 reacts with TCP1 alpha and another CCT subunit. In plants, ab90357 recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that ab90357 reacts with a subunit of a specialized chaperonin which folds phytochrome.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesab90357 reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, ab90357 reacts with TCP1 alpha and another CCT subunit. In plants, ab90357 recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that ab90357 reacts with a subunit of a specialized chaperonin which folds phytochrome.
  • ClonalityMonoclonal
  • Clone number91a
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-TCP1 alpha antibody [91a] images

  • IHC image of TCP1 alpha staining in Human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90357, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TCP1 alpha antibody [91a] (ab90357) at 1/1000 dilution

    Lane 1 : HELa cell lysate, heat shocked
    Lane 2 : PC12 cell lysate, heat shocked
    Lane 3 : 3T3 cell lysate, heat shocked
    Lane 4 : RK-13 cell lysate, heat shocked

    Developed using the ECL technique

    Predicted band size : 60 kDa
  • Overlay histogram showing HeLa cells stained with ab90357 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90357, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] ab18540, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TCP1 alpha antibody [91a] (ab90357)

This product has been referenced in:
  • Monzo K  et al. Proteomic analysis reveals CCT is a target of Fragile X mental retardation protein regulation in Drosophila. Dev Biol 340:408-18 (2010). Read more (PubMed: 20122915) »
  • Leroux MR & Candido EP Subunit characterization of the Caenorhabditis elegans chaperonin containing TCP-1 and expression pattern of the gene encoding CCT-1. Biochem Biophys Res Commun 241:687-92 (1997). WB ; Caenorhabditis elegans . Read more (PubMed: 9434769) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"