The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
ab10180 (10µg/ml) staining paraffin embedded Human Testis, microwaved antigen retreival with Tris/EDTA buffer pH9, HRP-staining.
References for Anti-TCP1 alpha antibody (ab10180)
This product has been referenced in:
Ryan T et al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem286:17060-8 (2011).
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