The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similaritiesBelongs to the TCP-1 chaperonin family.
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123508 at 6 µg/mg lysate for IP Subsequent Western blot detection used an antibody which recognized an downstream epitope of TCP1 alpha at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds
References for Anti-TCP1 alpha antibody (ab123508)
has not yet been referenced specifically in any publications.
Publishing research using ab123508? Please let us know so that we can cite the reference in this datasheet.
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