Recombinant
RabMAb

Anti-TCP1 alpha antibody [EPR4081] (ab92587)

Overview

  • Product name
    Anti-TCP1 alpha antibody [EPR4081]
    See all TCP1 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR4081] to TCP1 alpha
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human TCP1 alpha (UniProt ID: P17987).

  • Positive control
    • HeLa, ECV-304, A459 and 293T cell lysates, Human colon tissue, Human kidney tissue and HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
IP
  • Application notes
    ICC: 1/250 - 1/500.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • Sequence similarities
      Belongs to the TCP-1 chaperonin family.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    Images

    • All lanes : Anti-TCP1 alpha antibody [EPR4081] (ab92587) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : ECV-304 cell lysate
      Lane 3 : A459 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab92587 at 1/100 dilution staining TCP1 alpha in (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab92587 at 1/250 dilution staining TCP1 alpha in HeLa cells by Immunofluorescence.

    References

    ab92587 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92587.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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