Anti-TCP1 alpha antibody [EPR4081] (ab92587)

Overview

  • Product nameAnti-TCP1 alpha antibody [EPR4081]
    See all TCP1 alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR4081] to TCP1 alpha
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminal of Human TCP1 alpha (UniProt ID: P17987).

  • Positive control
    • HeLa, ECV-304, A459 and 293T cell lysates, Human colon tissue, Human kidney tissue and HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
IP
  • Application notesICC: 1/250 - 1/500.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
    • Sequence similaritiesBelongs to the TCP-1 chaperonin family.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI528772 antibody
      • c-cpn antibody
      • CCT alpha antibody
      • CCT antibody
      • CCT-alpha antibody
      • CCT1 antibody
      • Ccta antibody
      • CCTalpha antibody
      • D6S230E antibody
      • MGC133746 antibody
      • p63 antibody
      • T complex 1 antibody
      • T complex protein 1 alpha subunit antibody
      • T complex protein 1 antibody
      • T-complex homolog TCP1 antibody
      • T-complex protein 1 subunit alpha antibody
      • T-complex protein 1 subunit alpha B antibody
      • Tailless complex polypeptide 1 antibody
      • Tailless complex polypeptide 1A antibody
      • Tailless complex polypeptide 1B antibody
      • TCP 1 alpha antibody
      • Tcp-1 antibody
      • TCP-1-alpha antibody
      • TCP1 antibody
      • TCPA_HUMAN antibody
      • Tp63 antibody
      • TRic antibody
      see all

    Anti-TCP1 alpha antibody [EPR4081] images

    • All lanes : Anti-TCP1 alpha antibody [EPR4081] (ab92587) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : ECV-304 cell lysate
      Lane 3 : A459 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab92587 at 1/100 dilution staining TCP1 alpha in (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab92587 at 1/250 dilution staining TCP1 alpha in HeLa cells by Immunofluorescence.

    References for Anti-TCP1 alpha antibody [EPR4081] (ab92587)

    ab92587 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92587.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"