Anti-TCP1 beta antibody [EPR4083] (ab109184)

Overview

  • Product nameAnti-TCP1 beta antibody [EPR4083]
    See all TCP1 beta primary antibodies
  • Description
    Rabbit monoclonal [EPR4083] to TCP1 beta
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TCP1 beta aa 1-100. The exact sequence is proprietary.

  • Positive control
    • HeLa, MCF-7 and A431 cell lysates; Human kidney tissue
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4083
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similaritiesBelongs to the TCP-1 chaperonin family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 99D8.1 antibody
    • CCT 2 antibody
    • CCT beta antibody
    • CCT-beta antibody
    • CCT2 antibody
    • CCTB antibody
    • Chaperonin containing t complex polypeptide 1 beta subunit antibody
    • Chaperonin containing t complex polypeptide 1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 (beta) antibody
    • CTP:phosphocholine cytidylyltransferase 2 antibody
    • Epididymis secretory sperm binding protein Li 100n antibody
    • HEL S 100n antibody
    • MGC142074 antibody
    • MGC142076 antibody
    • MGC94480 antibody
    • PRO1633 antibody
    • T complex protein 1 beta subunit antibody
    • T complex protein 1 subunit beta antibody
    • T-complex protein 1 subunit beta antibody
    • TCP 1 beta antibody
    • TCP-1-beta antibody
    • TCPB_HUMAN antibody
    see all

Anti-TCP1 beta antibody [EPR4083] images

  • Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • All lanes : Anti-TCP1 beta antibody [EPR4083] (ab109184) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 57 kDa
  • ab109184, at 1/100 dilution, staining TCP1 beta in Human kidney tissue by Immunohistochemistry

References for Anti-TCP1 beta antibody [EPR4083] (ab109184)

ab109184 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"