Overview

  • Product name
    Anti-TCP1 beta antibody [EPR4084]
    See all TCP1 beta primary antibodies
  • Description
    Rabbit monoclonal [EPR4084] to TCP1 beta
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus of Human TCP1 beta.

  • Positive control
    • WB: HeLa, MCF-7 and Daudi cell lysates IHC-P: Human colon tissue, Human kidney tissue ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-TCP1 beta antibody (HRP) [EPR4084] (ab203463)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4084
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab92746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similarities
    Belongs to the TCP-1 chaperonin family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 99D8.1 antibody
    • CCT 2 antibody
    • CCT beta antibody
    • CCT-beta antibody
    • CCT2 antibody
    • CCTB antibody
    • Chaperonin containing t complex polypeptide 1 beta subunit antibody
    • Chaperonin containing t complex polypeptide 1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 antibody
    • Chaperonin containing TCP1 subunit 2 (beta) antibody
    • CTP:phosphocholine cytidylyltransferase 2 antibody
    • Epididymis secretory sperm binding protein Li 100n antibody
    • HEL S 100n antibody
    • MGC142074 antibody
    • MGC142076 antibody
    • MGC94480 antibody
    • PRO1633 antibody
    • T complex protein 1 beta subunit antibody
    • T complex protein 1 subunit beta antibody
    • T-complex protein 1 subunit beta antibody
    • TCP 1 beta antibody
    • TCP-1-beta antibody
    • TCPB_HUMAN antibody
    see all

Images

  • All lanes : Anti-TCP1 beta antibody [EPR4084] (ab92746) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 57 kDa
  • ab92746, at a 1/100 dilution, staining TCP1 beta in formalin fixed, paraffin embedded (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry. Detection: DAB staining.
  • ab92746, at a 1/100 dilution, staining TCP1 beta in HeLa cells by Immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab92746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92746, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Noormohammadi A  et al. Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan. Nat Commun 7:13649 (2016). WB ; Human . Read more (PubMed: 27892468) »
  • Shen W  et al. 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic Acids Res 43:4569-78 (2015). Read more (PubMed: 25855809) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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