• Product nameAnti-TCP1 delta antibody
    See all TCP1 delta primary antibodies
  • Description
    Rabbit polyclonal to TCP1 delta
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: AGLNPISTVT ELRNRHAQGE KTAGINVRKG GISNILEELV VQPLLVSVSA, corresponding to a region within C terminal amino acids 469-518 of Human TCP1 delta

  • Positive control
    • HepG2 cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab49151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
ICC/IF 1/200.
IHC-P 1/100.


  • RelevanceThe chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back to back rings, each containing 8 unique but homologous subunits (e.g. CCT3). CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin. TCP1 delta belongs to the TCP1 chaperonin family, and is a molecular chaperone; assisting the folding of proteins upon ATP hydrolysis. It is known to play a role, in vitro, in the folding of actin and tubulin.
  • Cellular localizationCytoplasmic > Cytoskeleton
  • Database links
  • Alternative names
    • CCT delta antibody
    • CCT4 antibody
    • CCTD antibody
    • chaperonin containing t-complex polypeptide 1, delta subunit antibody
    • chaperonin containing TCP1, subunit 4 (delta) antibody
    • SRB antibody
    • Stimulator of TAR RNA binding antibody
    • T complex protein 1 subunit delta antibody
    • TCP-1-delta antibody
    see all

Anti-TCP1 delta antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling TCP1 delta with ab49151 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TCP1. Right - Merge.

  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1µg/ml.
  • Immunocytochemistry/immunofluorescence analysis of Rat hippocampal neurons - 14DIV labeling TCP1 delta with ab49151 at 1/200. A Cy3-conjugated anti rabbit IgG (1/500) was used as the secondary antibody.

  • Predicted band size : 60 kDa
    Western blot analysis of HepG2 cell lysate labeling TCP1 delta with ab49151 at 1:1000. A Cy3-conjugated anti-rabbit IgG (1:10000) was used as the secondary antibody.
  • All lanes : Anti-TCP1 delta antibody (ab49151) at 1.25 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : molecular marker
    Lane 2 : HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

References for Anti-TCP1 delta antibody (ab49151)

This product has been referenced in:
  • Mallawaaratchy DM  et al. The phosphoinositide 3-kinase inhibitor LY294002, decreases aminoacyl-tRNA synthetases, chaperones and glycolytic enzymes in human HT-29 colorectal cancer cells. J Proteomics 75:1590-9 (2012). WB ; Human . Read more (PubMed: 22172953) »

See 1 Publication for this product

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