Overview

  • Product nameAnti-TCP1 epsilon antibody
    See all TCP1 epsilon primary antibodies
  • Description
    Rabbit polyclonal to TCP1 epsilon
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: NDGATILSMM DVDHQIAKLM VELSKSQDDE IGDGTTGVVV LAGALLEEAE , corresponding to a region within N terminal amino acids 72 - 121 of Human TCP1 epsilon (NP_036205)

  • Positive control
    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay:1/12500.

Target

  • RelevanceTCP1 epsilon is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP dependent manner. The complex folds various proteins, including actin and tubulin.
  • Cellular localizationCytoplasm; cytoskeleton and centrosome.
  • Database links
  • Alternative names
    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    see all

Anti-TCP1 epsilon antibody images

  • Anti-TCP1 epsilon antibody (ab82736) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-TCP1 epsilon antibody (ab82736) at 2 µg/ml

    Lane 1 : Mouse WT brain lysate
    Lane 2 : Rat brain lysate


    Predicted band size : 60 kDa

References for Anti-TCP1 epsilon antibody (ab82736)

ab82736 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"