• Product nameAnti-TCP1 epsilon antibody
    See all TCP1 epsilon primary antibodies
  • Description
    Rabbit polyclonal to TCP1 epsilon
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 492-541 (DCLHKGTNDM KQQHVIETLI GKKQQISLAT QMVRMILKID DIRKPGESEE) of human TCP1 epsilon (NP_036205).

  • Positive control
    • 293T cell lysate.



Our Abpromise guarantee covers the use of ab82778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.


  • RelevanceTCP1 epsilon is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP dependent manner. The complex folds various proteins, including actin and tubulin.
  • Cellular localizationCytoplasm; cytoskeleton and centrosome.
  • Database links
  • Alternative names
    • CCT 5 antibody
    • CCT epsilon antibody
    • CCT5 antibody
    • CCTE antibody
    • CCTepsilon antibody
    • Chaperonin containing T-complex polypeptide 1, subunit 5 antibody
    • Chaperonin containing TCP 1 epsilon antibody
    • Chaperonin containing TCP 1 subunit 5 antibody
    • Chaperonin containing TCP 1 subunit 5 epsilon antibody
    • Chaperonin containing TCP1 epsilon antibody
    • Chaperonin containing TCP1 subunit 5 antibody
    • Chaperonin containing TCP1 subunit 5 epsilon antibody
    • KIAA0098 antibody
    • T complex protein 1 epsilon subunit antibody
    • T complex protein 1 subunit epsilon antibody
    • TCP 1 epsilon antibody
    • TCP1epsilon antibody
    • TCPE antibody
    see all

Anti-TCP1 epsilon antibody images

  • Anti-TCP1 epsilon antibody (ab82778) at 1 µg/ml ( 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 20 kDa,24 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab82778 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82778, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TCP1 epsilon antibody (ab82778)

ab82778 has not yet been referenced specifically in any publications.

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