Anti-TCP1 theta antibody (ab96321)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TCP1 theta
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 111 - 337 of Human TCP1 theta.

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt-4 or Raji cell lysate. IF/ICC: HeLa cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/500.

Target

  • Relevance
    TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
  • Cellular localization
    Cytoplasm. Cytoplasm; cytoskeleton; centrosome
  • Database links
  • Alternative names
    • C21orf112 antibody
    • CCT 8 antibody
    • CCT theta antibody
    • CCT8 antibody
    • CCTQ antibody
    • Chaperonin containing TCP1 subunit 8 (theta) antibody
    • Chaperonin subunit 8 (Theta) (Predicted), isoform CRA_a antibody
    • Chaperonin subunit 8 (theta) antibody
    • Chromosome 21 open reading frame 112 antibody
    • D21S246 antibody
    • KIAA0002 antibody
    • PRED71 antibody
    • Renal carcinoma antigen NY REN 15 antibody
    • T complex protein 1 subunit theta antibody
    • T complex protein 1 theta subunit antibody
    • TCP 1 theta antibody
    • Tcpq antibody
    see all

Images

  • ICC/IF image of ab96321 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96321, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-TCP1 theta antibody (ab96321) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 60 kDa
    7.5% SDS PAGE
  • Immunohistochemistry analysis of paraffin-embedded OVCAR3 xenograft, using ab96321 at 1/500 dilution.

References

ab96321 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us with your question.

The TCP1 theta antibody ab96321 has already been tested in Western blot with human lysates, so we will fully guarantee it to work in this species and application. We do not have small samples of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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