Overview

  • Product nameAnti-TCP11L1 antibody
    See all TCP11L1 primary antibodies
  • Description
    Rabbit polyclonal to TCP11L1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 2-140 of Human TCP11L1.

  • Positive control
    • Human lymph node tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-TCP11L1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121231 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-TCP11L1 antibody (ab121231) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
  • ab121231, at 1/100 dilution, staining TCP11L1 in Human lymph node tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: Shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.

References for Anti-TCP11L1 antibody (ab121231)

ab121231 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"