Anti-TCP11L2 antibody (ab87197)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TCP11L2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (HQAFWDVLDS ELNADPPEFE HAIKLFEEIR EILLSFLTPG GNRLRNQICE) of Human TCP11L2 (NP_689985).

  • Positive control
    • Human fetal heart lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-TCP11L2 antibody images

  • Anti-TCP11L2 antibody (ab87197) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TCP11L2 antibody (ab87197)

ab87197 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Haematopoietic Cell Line)
Loading amount
30 µg
Specification
Haematopoietic Cell Line
Treatment
1uM PI-103 +- L-685 1uM for 72hr
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Clare Shepherd

Verified customer

Submitted Jul 31 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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