Anti-TCR alpha + TCR beta antibody [H57-597] (Phycoerythrin) (ab112230)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [H57-597] (Phycoerythrin)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityCommon epitope of the beta chain of the mouse alpha/beta T cell receptor (TCR) complex. This antibody does not crossreact with TCR gamma/delta type.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Affinity-purified DO-11.10 TCR.

  • Positive control
    • Mouse Strain: BALB/C (Splenic T cells, Thymus)
  • General notes


    ab112230 when used in an immobilized form was able to activate all alpha beta TCR bearing T cell hybridomas tested to produce IL-2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
    Note: EIA grade BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesab112230 when used in an immobilized form was able to activate all alpha beta TCR bearing T cell hybridomas tested to produce IL-2
  • ClonalityMonoclonal
  • Clone numberH57-597
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Anti-TCR alpha + TCR beta antibody [H57-597] (Phycoerythrin) images

  • Cell Source: Splenic T cells.
    ab112230 at a concentration of 1.0 µg/106.
    Percentage of Cells Stained Above Control: 89.3
    Isotypic Control: PE Hamster IgG
  • Cell Source: Thymus.
    ab112230 at a concentration of 1.0 µg/106.
    Percentage of Cells Stained Above Control: 75.2
    Isotypic Control: PE Hamster IgG

References for Anti-TCR alpha + TCR beta antibody [H57-597] (Phycoerythrin) (ab112230)

This product has been referenced in:
  • Kubo RT  et al. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol 142:2736-42 (1989). Read more (PubMed: 2467936) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"