Anti-TCR alpha + TCR beta antibody [14B11] (Biotin) (ab25202)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [14B11] (Biotin)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Syrian Hamster monoclonal [14B11] to TCR alpha + TCR beta (Biotin)
  • ConjugationBiotin
  • Specificityab25202 reacts with Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25202 binds to an epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants.
  • General notes


    ab25202 stains ~90% of NK1.1+CD3- splenocytes, and ~6% of CD8 + spleen cells from C57BL/6 mice. The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesab25202 stains ~90% of NK1.1+CD3- splenocytes, and ~6% of CD8 + spleen cells from C57BL/6 mice. The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
  • ClonalityMonoclonal
  • Clone number14B11
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

References for Anti-TCR alpha + TCR beta antibody [14B11] (Biotin) (ab25202)

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"