Anti-TCR alpha + TCR beta antibody [H57-597] (ab25336)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [H57-597]
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta
  • Specificityab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Othermore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)

  • General notes


    ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
  • ClonalityMonoclonal
  • Clone numberH57-597
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Other Use at an assay dependent concentration. In vitro stimulation of ab TCR-expressing T cells and In vitro and in vivo depletion of ab TCR-bearing cells.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

References for Anti-TCR alpha + TCR beta antibody [H57-597] (ab25336)

This product has been referenced in:
  • Shi YF  et al. In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes. J Immunol 146:3340-6 (1991). Read more (PubMed: 1827483) »
  • Murosaki S  et al. Failure of T cell receptor V beta negative selection in murine intestinal intra-epithelial lymphocytes. Int Immunol 3:1005-13 (1991). Read more (PubMed: 1661605) »

See all 8 Publications for this product

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Thank you for contacting us yesterday in regards to the anti-TCR alpha + TCR beta antibody [H57-597] (ab25336).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"