Anti-TCR alpha + TCR beta antibody [H57-597] (Allophycocyanin) (ab25324)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [H57-597] (Allophycocyanin)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • SpecificityThis antibody does not react with gamma delta TCR bearing cells.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    TCR affinity purified from mouse T cell hybridoma DO-11.10.

  • General notes


    This antibody, in plate bound or soluble form, activates alpha beta TCR bearing T cells. In vitro and in vivo, this antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete alpha beta TCR bearing cells to near completion thus preventing graft rejection. The antibody has also been used for in vitro depletion studies.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesThis antibody, in plate bound or soluble form, activates alpha beta TCR bearing T cells. In vitro and in vivo, this antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete alpha beta TCR bearing cells to near completion thus preventing graft rejection. The antibody has also been used for in vitro depletion studies.
  • ClonalityMonoclonal
  • Clone numberH57-597
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

References for Anti-TCR alpha + TCR beta antibody [H57-597] (Allophycocyanin) (ab25324)

ab25324 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"