Anti-TCR alpha + TCR beta antibody [H57-597] (FITC) (ab25010)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [H57-597] (FITC)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityCommon epitope of the ß chain of the mouse aß T cell receptor (TCR) complex
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    TCR affinity purified from T-cell hybridoma DO-11.10 (Mouse)

  • General notes


    ab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Primary antibody notesab25010 can be used for in vitro stimulation of alpha beta TCR-expressing T cells and in vitro and in vivo depletion of aß TCR-bearing cells. Plate-bound or soluble ab25010 activates aß TCR bearing T cells.
  • ClonalityMonoclonal
  • Clone numberH57-597
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Anti-TCR alpha + TCR beta antibody [H57-597] (FITC) images

  • ab25010, Hamster monoclonal [H57-597] to TCR alpha + TCR beta (FITC), staining mouse (BALB/c) splenocytes by flow cytometry. 1µg antibody was used / 106 cells. The cells were double stained with a PE-conjugated rat anti-mouse CD19 and then analysed on a FACScan flow cytometer.

References for Anti-TCR alpha + TCR beta antibody [H57-597] (FITC) (ab25010)

This product has been referenced in:
  • Kubo RT  et al. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol 142:2736-42 (1989). Read more (PubMed: 2467936) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"