Anti-TCR alpha + TCR beta antibody [H57-597] (PE/Cy5®) (ab95762)

Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [H57-597] (PE/Cy5®)
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • SpecificityCommon epitope of the beta chain of the mouse alpha/beta T cell receptor (TCR) complex. This antibody does not crossreact with TCR gamma/delta type.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Affinity purified TCR from Mouse DO-11.10 cells.

  • Positive control
    • C57Bl/6 splenocytes; mouse thymocyte and splenocyte suspensions

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberH57-597
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on a test volume of 100 µl).

Ab157805-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

ab157805-Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • FLJ22602 antibody
    • MGC117436 antibody
    • MGC22624 antibody
    • MGC23964 antibody
    • MGC71411 antibody
    • T cell antigen receptor alpha polypeptide antibody
    • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
    • T cell receptor alpha (V D J C) antibody
    • T cell receptor alpha chain C region antibody
    • T cell receptor alpha constant antibody
    • T cell receptor alpha locus antibody
    • T cell receptor beta cluster antibody
    • T cell receptor beta locus antibody
    • TCRA antibody
    • TCRB antibody
    • TCRD antibody
    • TRA antibody
    • TRAC antibody
    • TRB antibody
    see all

Anti-TCR alpha + TCR beta antibody [H57-597] (PE/Cy5®) images

  • Flow cytometry staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of ab95762 (filled histogram). Total cells were used for analysis.

References for Anti-TCR alpha + TCR beta antibody [H57-597] (PE/Cy5®) (ab95762)

ab95762 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"