Overview

  • Product nameAnti-TCR alpha + TCR beta antibody [R73]
    See all TCR alpha + TCR beta primary antibodies
  • Description
    Mouse monoclonal [R73] to TCR alpha + TCR beta
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation - Rat T blasts and Rat erythrocytes.

  • Positive control
    • LOU Rat splenocytes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberR73
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
IHC-Fr
  • Application notesFlow Cyt: Use 0.25 µg for 106 cells and use a 100 µl total staining volume
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • FLJ22602 antibody
      • MGC117436 antibody
      • MGC22624 antibody
      • MGC23964 antibody
      • MGC71411 antibody
      • T cell antigen receptor alpha polypeptide antibody
      • T cell antigen receptor beta polypeptide T cell receptor beta cluster antibody
      • T cell receptor alpha (V D J C) antibody
      • T cell receptor alpha chain C region antibody
      • T cell receptor alpha constant antibody
      • T cell receptor alpha locus antibody
      • T cell receptor beta cluster antibody
      • T cell receptor beta locus antibody
      • TCRA antibody
      • TCRB antibody
      • TCRD antibody
      • TRA antibody
      • TRAC antibody
      • TRB antibody
      see all

    Anti-TCR alpha + TCR beta antibody [R73] images

    • Staining of LOU Rat splenocytes with ab91121 at 0.125 µg (blue histogram), or purified Mouse IgG1k, isotype control at 0.5 µg (green histogram), followed by treatment with FITC anti-mouse IgG. Total viable cells were used for analysis.

    References for Anti-TCR alpha + TCR beta antibody [R73] (ab91121)

    This product has been referenced in:
    • Hünig T  et al. A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med 169:73-86 (1989). Read more (PubMed: 2783336) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"